Genetic Engineering News The chromatographic column was an UPLC BEH C18 50 × 2.1 mm, 1.7 μm particle size (Waters) with a mobile phase A containing 0.1% formic acid in water and a mobile phase B containing 0.1% formic acid in acetonitrile . The flow rate was set at 0.4 mL/min ...
Genetic Engineering News Aside from water, acetonitrile is arguably the most used solvent in HPLC. It has become prohibitively expensive in recent years; users pay “both ways” for the luxury of using the overwhelming majority of solvents. A four-liter bottle of HPLC-grade ...
